Updated: Sep 04, 2026
No. of Questions: 562 Questions & Answers with Testing Engine
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| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
|---|---|
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Real Exam Qty: | 100 |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions |
| Related Certifications: | MLT(ASCPi) MLS(ASCP) PBT(ASCP) |
| Passing Score: | 400 (scale 0-999) |
| Available Languages: | English |
| Exam Duration: | 150 minutes |
| Recommended Training: | ASCP Recommended Reading List ASCP BOC MLT Content Guideline |
| Exam Registration: | ASCP BOC Official Application Portal Pearson VUE Testing Center Scheduling |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
| Section | Weight | Objectives |
|---|---|---|
| Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies - Infectious disease serology - Immune system principles and components |
| Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes |
| Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Compatibility testing - Antibody detection and identification - Blood components and storage - Transfusion reactions and adverse events |
| Microbiology | 15-20% | - Specimen collection and culture techniques - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms |
| Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis |
| Laboratory Operations | 5-10% | - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
Question 1
Bacitracin susceptibility is a presumptive test for the identification of Lancefield group A Streptococcus.
Which of the following is a presumptive test for the identification of Lancefield group A Streptococcus:
A. Gram stain
B. Hippurate hydrolysis
C. Bacitracin susceptibility
D. Positive coagulase
Question 2
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell and a swollen vesicle in a fungus culture are excellent clues indicative of Aspergillus. Aspergillus has the ability to infect primarily immunocompromised hosts, and causes pneumonia and/or disseminates to other organs. Clinical specimens should be inoculated onto primary isolation media, such as Sabouraud's dextrose agar. Aspergillus spp. are fast growing and can be white, yellow, yellow-brown, brown to black or green in color.
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell and a swollen vesicle in a fungus culture are excellent clues indicative of:
A. Aspergillus
B. Acremonium
C. Penicillium
D. Paecilomyces
E. Scopulariopsis
Question 3
Twelve weeks after onset of the disease, patients with uncomplicated acute hepatitis B usually will demonstrate which of the following in their serum?
A. HBsAg
B. Anti-HBe
C. Anti-HIV
D. Anti-HTLV
Question 4
Provide the equivalent measurement for one pint.
A. 0.25 liters
B. 4.2 liters
C. 0.474 liters
D. 0.1 liter
Question 5
Parathyroid hormone regulates serum calcium by acting on bone, kidney, and intestines while regulating phosphate by stimulating the intestines and the kidneys, enhancing absorption and reabsorption respectively.
Chem
The parathyroid hormone is important in the regulation of:
A. acid and alkaline phosphatase
B. calcium and phosphate
C. iodine and bromine
D. zinc and magnesium
Solutions:
| Question 1 Answer: C | Question 2 Answer: A | Question 3 Answer: B | Question 4 Answer: C | Question 5 Answer: B |
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