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Updated: Sep 04, 2026

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ASCP ASCP-MLT Exam Overview:

Certification Vendor:ASCP Board of Certification (ASCP BOC)
Exam Name:MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
Exam Number:ASCP-MLT
Exam Price:$235 USD (standard); $215 USD (reduced for eligible regions)
Certificate Validity Period:3 years (renewable via CMP)
Real Exam Qty:100
Exam Format:Computer Adaptive Testing (CAT), Multiple-choice questions
Related Certifications:MLT(ASCPi)
MLS(ASCP)
PBT(ASCP)
Passing Score:400 (scale 0-999)
Available Languages:English
Exam Duration:150 minutes
Recommended Training:ASCP Recommended Reading List
ASCP BOC MLT Content Guideline
Exam Registration:ASCP BOC Official Application Portal
Pearson VUE Testing Center Scheduling
Sample Questions:ASCP ASCP-MLT Sample Questions
Exam Way:Proctored computer-based testing at authorized Pearson VUE test centers
Pre Condition:Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available
Official Syllabus URL:https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT

ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Immunology and Serology5-10%- Autoimmune and immunodeficiency disorders
- Immunologic testing methodologies
- Infectious disease serology
- Immune system principles and components
Clinical Chemistry20-25%- Endocrinology and therapeutic drug monitoring
- Toxicology and liver/kidney function tests
- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
Hematology20-25%- White blood cell morphology and diseases
- Red blood cell physiology and disorders
- Hemostasis and coagulation testing
- Routine and automated hematology procedures
Blood Banking (Immunohematology)15-20%- Blood group systems and antigens
- Compatibility testing
- Antibody detection and identification
- Blood components and storage
- Transfusion reactions and adverse events
Microbiology15-20%- Specimen collection and culture techniques
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility
- Identification of pathogenic microorganisms
Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Semen and amniotic fluid testing
- Cerebrospinal, serous, and synovial fluids
- Physical, chemical, and microscopic analysis
Laboratory Operations5-10%- Quality control and quality assurance
- Pre-analytical, analytical, and post-analytical processes
- Laboratory mathematics and instrumentation
- Laboratory safety and regulatory compliance

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

Question 1

Bacitracin susceptibility is a presumptive test for the identification of Lancefield group A Streptococcus.
Which of the following is a presumptive test for the identification of Lancefield group A Streptococcus:

A. Gram stain
B. Hippurate hydrolysis
C. Bacitracin susceptibility
D. Positive coagulase


Question 2

The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell and a swollen vesicle in a fungus culture are excellent clues indicative of Aspergillus. Aspergillus has the ability to infect primarily immunocompromised hosts, and causes pneumonia and/or disseminates to other organs. Clinical specimens should be inoculated onto primary isolation media, such as Sabouraud's dextrose agar. Aspergillus spp. are fast growing and can be white, yellow, yellow-brown, brown to black or green in color.
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell and a swollen vesicle in a fungus culture are excellent clues indicative of:

A. Aspergillus
B. Acremonium
C. Penicillium
D. Paecilomyces
E. Scopulariopsis


Question 3

Twelve weeks after onset of the disease, patients with uncomplicated acute hepatitis B usually will demonstrate which of the following in their serum?

A. HBsAg
B. Anti-HBe
C. Anti-HIV
D. Anti-HTLV


Question 4

Provide the equivalent measurement for one pint.

A. 0.25 liters
B. 4.2 liters
C. 0.474 liters
D. 0.1 liter


Question 5

Parathyroid hormone regulates serum calcium by acting on bone, kidney, and intestines while regulating phosphate by stimulating the intestines and the kidneys, enhancing absorption and reabsorption respectively.
Chem
The parathyroid hormone is important in the regulation of:

A. acid and alkaline phosphatase
B. calcium and phosphate
C. iodine and bromine
D. zinc and magnesium


Solutions:

Question 1
Answer: C
Question 2
Answer: A
Question 3
Answer: B
Question 4
Answer: C
Question 5
Answer: B

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